Development -- Hiremath et al. 134 (20): 3703 Figure IG7

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Fig. 7. Northern blot analysis of milk protein RNA levels in 26-week-old virgin female mice. 28S and 18S rRNA subunits visualized by ethidium bromide staining provided loading controls. In addition, K18 (Krt18), a luminal cell marker, was used as a positive control for the integrity of the RNA and epithelial content. Three mice of each genotype were examined as indicated: PR^+/- (lanes 2-4), PR^+/-; ${Delta}$ N89ß-catenin (lanes 8-10), PR^-/-; ${Delta}$ N89ß-catenin (lanes 11-13) and PR^-/- (lanes 5-7). RNA from lactating (L, lane 14) and virgin (V, lane 1) mice were used as positive and negative controls, respectively.