Fig. 2. D-V compartmentalization of limb mesenchyme. (A-H) lacZ staining of mouse embryos after 4OHTM injection at different gestational stages and recovery at various days of development, as indicated. (A,B) Dorsal and lateral views, respectively, of a large clone induced at E8.0. (C-H) Examples of D-V clone distribution at different embryonic stages, as indicated. The arrowhead in D indicates a local violation of the D-V restriction. (I) Bar chart indicating the position of the boundary respected by dorsal (left, red) and ventral (right, blue) clones, expressed as the percentage of the total D-V distance from dorsal. The x-axis indicates the percentage of specimens from each class.