Fig. 2. Transcription patterns of npc2-like genes ascertained with in situ hybridization in Drosophila melanogaster. npc2a is deposited maternally (A) and is broadly expressed with a higher level of expression in many tissues, including the midgut (arrow in B), salivary gland (arrow in C) and ventral nerve cord (arrow in D). (E) npc2a in situ staining signal was not detected in the homozygous npc2a mutant embryos. (F) The salivary gland expression of npc2d. (G,H) npc2g was specifically expressed in head mesoderm (arrow in G) and fat body (arrow in H). (I) npc2b was specifically expressed in trachea (arrow) and hypopharynx (arrowhead). (J) npc2b in situ staining signal was not detected in the homozygous npc2b mutant. (K,L) npc2a and npc2b were expressed in larval brain hemispheres and ring gland (arrows), respectively.