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Figure 1


Fig. 1. Defective vasculogenesis in Ihh-/- yolk sac is associated with reduced Foxf1 expression. (A-C) Comparison of yolk sac vasculature in E9.5 wild-type (wt) (A), Ihh-/- (B) and Foxf1-/- (C) mouse embryos. (D-G) Whole-mount in situ hybridization of E8.5 wt (D,F) and Ihh-/- (E,G) embryos with a Foxf1 probe. F and G are magnified views of the yolk sac surface of the embryos in D and E, respectively. Foxf1 expression in yolk sac blood islands is seen as diffuse blue speckles against the pale background of the out-of-focus embryo heads, and is much weaker in the Ihh mutant.