Fig. 2. Ida functions specifically to control the cortical localisation of Miranda and its associated proteins, but not to control PON/Numb. (A-G) Wild-type localisation of Inscuteable (Insc, green; A,B), Prospero (Pros, green; C), Staufen (Stauf, green; D), Brat (green; E), Numb (green; F), PON (green; G) and Miranda (Mira, red; A-G) in mitotic larval neuroblasts (NBs). (H,I) Inscuteable (green) localisation is unaffected in ida mutant NBs and a cortical Inscuteable crescent is always seen. Where Miranda (red in H',I') crescents form, Inscuteable and Miranda crescents occupy opposite sides of the cell cortex (I,I'). (J-L) Prospero (green, J), Staufen (green, K) and Brat (green, L) accumulate pericentrosomally with Miranda (J'-L') in ida mutant NBs. (M,N) By contrast, Numb (green, M) and PON (green, N) remain associated with the NB cortex even in ida mutant NBs that show strong Miranda mislocalisation defects (M',N'). Miranda localisation is shown in red (H'-N'); DNA in blue. Scale bar: 10 µm.