Fig. 4. Robo is required cell-autonomously in the aCC during dendrogenesis. (A,B) Tracings of robo⁴/robo⁴ (A) and robo¹/robo⁴ aCCs with cell-specific genetic rescue (robo⁴/robo¹;eve'-GAL4^RN2/UAS-robo^WT: B) at hour 17. (C) Relative sizes of aCC dendrites [measured as mean±s.e.m. number of dendritic tips per neuron, with the wild-type (+/+; value of 15.1) as 100%] in +/+ (n=14), cell-specific RNAi (n=15), robo⁴/robo⁴ and robo¹/robo⁴ (n=14), rescue (n=7), robo⁴/+ (n=6), 1x overexpression (eve'-GAL4^RN2/UAS-robo^WT; n=11), and 2x overexpression (eve'-GAL4^RN2/UAS-robo^WT, UAS-robo^WT; n=6). Asterisks indicate P<0.01 by two-tailed t-test against wild type. Scale bar: 5 µm.