Fig. 7. Expression of a dominant-negative Spalt4 constructs in the otic placode reduces the size of the otic vesicle. (A) Whole mount and (B) section of an embryo electroporated with the control plasmid and hybridized in situ with a Sox10 RNA probe. (C) Whole mount and (D) section of an embryo electroporated with the dominant-negative Spalt4 plasmid and hybridized in situ with a Sox10 RNA probe. (E) Embryo 8 hours post-electroporation with control plasmid and analyzed with GFP. (F) Same section as in E analyzed for cell death with TUNEL. (G) Embryo 8 hours post-electroporation with dominant-negative Spalt4 plasmid and analyzed with GFP. (H) Same section as in G analyzed for cell death with TUNEL. Scale bars: 200 µm (A,C) 100 µm (B,D).