Fig. 6. Tead4^-/- ES cells appear similar to and express the same markers as wild-type cells. (A) Tead4^+/+ and Tead4^-/- ES cells generated from E2.5 embryos at 10x and 40x magnification. (B) ES cells derived from embryos produced from Tead4 heterozygous matings were genotyped for Tead4. (C) Semi-quantitative RT-PCR of RNA from Tead4^+/+ and Tead4^-/- ES cells for markers of pluripotentcy including Oct4, Fgf4, Nanog, Sox2 and Rex1, and differentiation [brachyury (T) at 30 cycles], with Gapdh as a reference. (D) Semi-quantitative RT-PCR analysis of embryoid bodies generated from Tead4^+/+ and Tead4^-/- ES cells at days 0, 1, 2, 4 and 5. Differentiation markers include Rex1 for undifferentiated ES cells, brachyury (T) (at 35 cycles) for mesoderm, Hnf4a and Gata6 for endoderm, and Fgf5 for ectoderm, with Gapdh as a reference control. W, wild type; M, mutant.