Fig. 7. Tead4^-/-;Meox2-Cre/+ mice were viable despite the absence of Tead4 mRNA. (A) PCR genotyping of mouse tail samples from matings of Tead4^lox/lox and Tead4^+/-;Meox2-Cre⁺ animals. (B) Analysis of Tead4 mRNA expression in major tissues isolated from wild-type and Tead4^-/-;Meox2-Cre⁺ mice by RT-PCR. The Tead4 PCR was performed using 37 cycles with Gapdh as a reference (at 35 cycles). W, wild type; L, loxP; M, mutant.