Fig. 3. A wave of exocytosis during anaphase I. C. elegans embryos expressing histone::GFP and labeled with the plasma membrane dye FM2-10 were imaged using SFC every 200 milliseconds. After the chromosome separation initiated (A), exocytic events (arrows) occurred near the spindle (B) and spread across the cortex (C,D) before the completion of anaphase I. Images in C and D are maximum projection summations of several frames in which a vesicle fusion event occurs. A gap is formed between the plasma membrane and the vitelline layer near the polar body (E, bracket). (F) Images of the plasma membrane before and after exocytosis, labeled with FM2-10 or a fluorescent dextran. Scale bar: 10 µm.