Fig. 6. The dynamics of the exocytic wave during anaphase I. C. elegans embryos labeled with histone::GFP and FM2-10 were imaged every 333 milliseconds using MPLSM. (A) Total exocytic events observed in wild-type, chs-1(RNAi) and cks-1(RNAi) embryos were nearly twice that of sep-1(e2406) and sep-1(RNAi) embryos. (B) Kinetic profiles of chromosome separation (plotted as a line) and exocytic events (columns) from single embryo recordings representative of average dynamics. Images from the movies are shown in C-K; brackets indicate distance measured between chromosomes, arrows indicate quantitated exocytic events. In wild type (green), degranulation initiated after chromosomes separated by 1.5 µm, and completed before polar body extrusion. In sep-1(RNAi) (red), chromosome separation was severely reduced, the wave of exocytosis was reduced and took much longer. By contrast, in sep-1(e2406) embryos (blue) chromosomes initially separated normally, but still had a reduced level of exocytosis that began after chromosomes separated by 2.5 µm. Scale bar: 10 µm.