Fig. 8. Separase co-localization during meiosis I in C. elegans. During prometaphase I, the outer kinetochore protein, HIM-10 (A, red), co-localized in cortical filaments with separase (B, green); merge in (C). During anaphase I, cortical granules labeled with WGA (D, red) are also labeled with separase (E, green); merge in F, maximum projection image of four cortical planes. A sep-1(e2406) mutant embryo in anaphase I had cortical granules labeled with WGA (G, red) that were not labeled with separase antibody (H, green), although separase was still present but reduced on the anaphase spindle (arrow); merge in I. Scale bar: 10 µm.