Fig. 6. Spatial relationship between PIN1, PID and MAB4/ENP in cultured Arabidopsis cells. (A,C) PIN1-GFP was expressed under the control of its own promoter in Arabidopsis protoplasts (A). GFP-PID was expressed under the control of the CaMV 35S promoter in Arabidopsis protoplasts (C). GFP fluorescence images (left) and Nomarski image (right) were taken with an epifluorescence microscope. (B,D) Localization of PIN1-GFP (B), GFP-PID (D) (green; left) and mRFP-MAB4/ENP (red; middle). mRFP-MAB4/ENP and PIN1-GFP or GFP-PID were co-introduced into Arabidopsis protoplasts. Merged image (right) of mRFP-MAB4/ENP, and PIN1-GFP (B) and GFP-PID (D). An arrowhead in B indicates the close proximity of the PIN1-GFP and mRFP-MAB4/ENP florescence, which is shown enlarged in the inset. Arrows in D indicate the colocalization of GFP-PID and mRFP-MAB4/ENP florescence, which is shown enlarged in the inset. Scale bars: 10 µm in A-D.