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Figure 7


Fig. 7. Subcellular localization of MAB4/ENP. (A-D) Localization of MAB4/ENP-GFP (green; left) and mRFP or Venus-tagged subcellular marker genes (red; middle). MAB4/ENP-GFP and mRFP- or Venus-subcellular markers were co-introduced under the control of the CaMV 35S promoter into Arabidopsis protoplasts. Merged image (right) of MAB4-GFP and the late endosome marker ARA6-mRFP (A), the early endosome marker mRFP-ARA7 (B), the cis-Golgi marker Venus-SYP31 (C) and the trans-Golgi-network (TGN) marker Venus-SYP41 (D). Arrows in A indicate colocalization of fluorescence of MAb4/ENP-GFP and ARA6-mRFP. Scale bars: 10 µm in A-D.