Fig. 7. Interaction of Nodal with sulfated GAGs. Recombinant mouse Nodal was incubated with heparin-sepharose beads, which were then isolated and subjected to stepwise elution with NaCl (A) or with heparin, CS or HS (B). Input (IP), flow through (FT), washed (1-3) and eluted fractions were subjected to immunoblot analysis with antibodies to Nodal. The amount of Nodal in the various fractions obtained by elution with NaCl (normalized relative to that in the fraction containing the most Nodal) was quantitated by densitometry of the immunoblot shown.