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Figure 3


Fig. 3. bro-1 rnt-1 double mutants have a similar phenotype to single mutants. (A) him-8(e1489) male tail. (B) bro-1(tm1183) rnt-1(tm388); him-5(e1490) male tail. White arrowheads indicate four rays visible in this animal. (C) V lineage traces up to mid L3, omitting the L1 asymmetric division. The symbols used for different cell types are the same as for Fig. 2. (Ci) WT hermaphrodite. (Cii) bro-1(tm1183) rnt-1(tm388) hermaphrodite. (D) Bar chart showing seam cell number in him-5(e1490) hermaphrodites carrying SCM::GFP (strain AW60; n=24; effectively WT), bro-1(tm1183); him-5(e1490) SCM::GFP hermaphrodites (strain AW186; n=37), rnt-1(tm388); him-5(e1490) SCM::GFP hermaphrodites (strain AW187; n=53) and bro-1(tm1183) rnt-1(tm388); him-5(e1490) SCM::GFP hermaphrodites (strain AW180; n=47). (E) Bar chart showing ray number in him-8(e1489) males (n=102), bro-1(tm1183); him-8(e1489) males (n=94), rnt-1(tm388); him-8(e1489) males (n=84) and bro-1(tm1183) rnt-1(tm388); him-8(e1489) males (n=97). (F) Bar chart showing seam cell number in the adult hermaphrodite progeny of him-5(e1490) and bro-1(tm1183); him-5(e1490) mutant animals that had been either subjected to RNAi of the cki-1 gene (cki-1, n=23; bro-1; cki-1, n=24) or injected with TE only as a control (WT, n=21; bro-1, n=21).