Fig. 4. BRO-1 and RNT-1 are largely co-expressed. (A-C) Animals carrying an integrated bro-1::GFP reporter construct (dpy-20 (e2017)IV; msIs344[pHK196 bro-1::GFP + pMH86]) (strain YK149). (A) Larva around the L1-L2 molt. Most seam cells (labelled) are undivided in this animal except V2.p and V3.p. Faint expression is also observed in some hyp7 nuclei (white arrowhead). (B) BRO-1::GFP in the seam and uterine seam (utse) in adult hermaphrodite, after fusion of these compartments in L4. (C) L3 larva in which V1.p-derived seam cells have over-proliferated (extra nuclei are indicated by the white oval outline). Seam cells yet to divide are indicated by white arrowheads. Faint hyp7 expression is again observed in nuclei of both embryonic and larval origin (all unlabelled nuclei). (D) WT larva around the L1-L2 molt carrying both bro-1::DsRed and rnt-1::GFP translational reporter constructs (strain AW192, ouEx43). Co-expression can be clearly observed in all 10 seam cell nuclei, which have yet to divide in L2 (labelled, fluorescence appears yellow). BRO-1::RFP, but not RNT-1::GFP expression, is also observed in hypodermal nuclei (white arrowheads) and certain pharyngeal neurons (white arrow). (E) Higher magnification image (x1.33) of a different focal plane of region of specimen in D showing co-localisation of BRO-1::RFP and RNT-1::GFP in certain body wall muscle nuclei (white arrowheads). Other body wall muscle nuclei look green, and are therefore expressing rnt-1 only (white arrows). (F) WT young adult showing fully formed vulva (white arrowhead). (G) bro-1(tm1183) rnt-1(tm388) adult hermaphrodite that has ruptured at the vulva at the L4-adult molt (white arrowhead). (H) Table showing penetrance of vulval defects in N2 WT, rnt-1(tm388), bro-1(tm1183) and bro-1(tm1183) rnt-1(tm388) hermaphrodites. Rup, ruptured vulva; Pvl, protruding vulva; Bag, `bag of worms' phenotype due to hatching of eggs inside mother.