Fig. 6. BRO-1 represses rnt-1 expression. (A) rnt-1::GFP expression (msIs114[pHK192 + rol-6]) in a bro-1(tm1183) L1 larva rescued with bro-1::RFP (msEx446[pHK328]) (strain YK153). (B) L1 larva of the same strain, but this animal has lost the bro-1::RFP rescuing array. Both images were taken under identical exposure conditions. It was not possible to observe adult hermaphrodites carrying a rnt-1::GFP transgene and bro-1(tm1183) mutation because these animals invariably die during late larval development as a result of rupture of the vulva. Hence the strain used in these experiments consisted of an integrated rnt-1::GFP transgene in a bro-1(tm1183) mutant background kept alive by extrachromosomal rescuing bro-1::RFP. (C) RT-PCR analysis of rnt-1 transcripts in WT and bro-1(tm1183) animals. ama-1 is an internal control. The smaller, fainter band is an alternatively spliced variant of rnt-1 (T. Braun and A.W., unpublished).