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Figure 1


Fig. 1. HIF1{alpha} is expressed by hypoxic differentiating chondrocytes. (A) Sections of E12.5 autopod showing avascular cartilage. The vasculature was detected by immunofluorescence using anti-CD31 antibody (green); cartilage was detected by anti-collagen type II (red). (B,C) Sections through the elbow show hypoxic cells in the condensed mesenchyme at E11.5 (B) and E12.5 differentiating chondrocytes (C). (D,E) Section through the autopod at E12.5 (D) and E13.5 (E). Hypoxic cells were detected by immunofluorescence using Hypoxyprobe-1. (F) HIF1 detection in E13.5 digits by immunofluorescence using anti-HIF1{alpha} antibody. (G-J) Pgk1 expression was detected by in situ hybridization on sections, showing the humerus region (G) and the digits (I) of E12.5 control. The expression was lost in humerus, ulna (H) and digits (J) of Prx1-Cre-Hif1{alpha} autopods. Black arrows indicate Pgk1 expression in the interzone; red arrows indicate Pgk1 expression in the inner part of the digit. h, humerus; r, radius; u, ulna.