Development -- Amarilio et al. 134 (21): 3917 Figure IG6

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Fig. 6. Vegf expression and vasculature formation in Hif1 ${alpha}$ -deleted limb. Immunofluorescence of E12.5 sections of control (A) and Prx1-Cre-Hif1 ${alpha}$ (B) mouse limbs using antibodies for collagen type II (red) and CD31 (green). (C) Quantitative RT-PCR demonstrates reduction in Vegf mRNA level in Prx1-Cre-Hif1 ${alpha}$ E12.5 limbs (n=2).