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Figure 7


Fig. 7. HIF1{alpha} is necessary for mesenchymal cell differentiation under hypoxia. (A,B) Micromass culture of mesenchymal cells derived from E11.5 Hif1{alpha} floxed/floxed mouse embryos were infected with AdCre or AdGfp (control) and were cultured under normoxic (20% O2) or hypoxic (1% O2) conditions as indicated (gray, AdGfp; black, AdCre). Quantitative RT-PCR shows deletion of Hif1{alpha} (A) and loss of Pgk1 induction under hypoxia (B) in AdCre-infected cells relative to AdGfp-infected cells. (C) Alcian Blue staining (above) and immunohistochemical staining for collagen type II (Col2a1; below) reveal reduction in cartilage nodule formation in AdCre-infected cells cultured under hypoxia. (D,E) Substantial reduction in collagen II (D) and aggrecan (E) mRNA is detected by quantitative RT-PCR (n=3).