Fig. 1. Plcz1 expression in wild-type and transgenic mice. (A) Plcz1 RT-PCR on testes (t) and brains (br) from duplicate wild-type males at the ages shown (upper) and from wild-type (wt) and F₂ transgenic females on brain (br), heart (h), lung (lu), liver (li), spleen (sl), kidney (k), skeletal muscle (sm) and ovaries (o) (beneath). (B) Configurations of Plcz1 promoter-mapping constructs with Cre and rPLCZ1 reporters, showing cAMP-responsive element binding protein (CREBP) cognate sequences (green), the putative transcriptional start site (red, boxed), a region of high Z-DNA potential (bold, italicised) and the putative translational start codon (red, underlined). Sequences unique to each construct are indicated in blue, with Cre uppermost. Beneath are shown the structures of downstream open reading frames. (C) Structures (not to scale) of CV and CS tg constructs. Arrows mark the start and direction of translation. (D) Immunoblotting to show rPLCZ1 and PLCZ1 expression in heart (h), skeletal muscle (sm), testes (t) and ovaries (o) of CS and CV hemizygotes (tg) and aged-matched, non-transgenic littermates (wt).