Fig. 3. Timing and early in vivo consequences of rPLCZ1 expression. (A) Epifluorescence microscopy of mouse ovarian follicle section showing Venus expression (left) within a maturing primary oocyte (arrowhead), stained for DNA (right). (B) Asynchronous parthenogenotes recovered from hemizygotes 16 hours post-hCG. (C) Hematoxylin and Eosin staining of an 11-week-old hemizygous ovary which appeared macroscopically normal, at low and higher (inset) magnification, showing a nascent follicular choriocarcinoma or yolk sac tumour. Scale bars: 50 µm in A,C; 20 µm in B.