Fig. 4. Ovarian tumours in PLCZ1-expressing female mice. (A) Hemizygous F₂ CV3 and CS16 females at 6 months, with age-matched control (wt) showing abdominal distensions (arrowheads) and the tumours that caused them. (B) Percentages of transgenic females with tumours at the ages shown. (C) Reproductive tissue from a hemizygote at 6 months with a small tumour (arrowhead) and three regressing implantation fossa (arrows). (D) Dosages of tgs (rPlcz1) determined for 12 ovarian tumours relative to levels of the native genes Actb and H2afz. All tumours were from hemizygous females. Somatic tissue from homozygotes (CS1006h and CS1008h) gave expected tg dosages of 2. Error bars (±s.e.m.) were produced from results obtained for different (n) samples from the same tumour. Discrete portions from the tumour CS832-1 (bracketed) possessed disparate relative tg dosages. Scale bars: 1 cm.