Fig. 5. Plcz1 expression in tumours and in the tumourigenic strain LT/Sv, and expression of its human orthologue in tumours. (A) Relative mRNA levels determined by qPCR for tumours from CV3 (stippled) and CS16 (grey) relative to wild-type ovary, or combined and expressed relative to skeletal muscle (white). Error bars, ±s.e.m.; ^*, not detected. Corresponding P-values are shown for unpaired Student's t-tests. (B) Tumour transcript levels of maternally- (white) and paternally- (grey) expressed imprinted genes determined by qPCR and represented relative to respective levels in wild-type ovary (1.0). Error bars, ±s.e.m. P-values (above), unpaired Student's t-tests. (C) RT-PCR analysis of two CV3 (CV) and four CS16 (CS) tumours, wild-type placenta (pl) and ovaries (o), for placental marker and metastasis-associated gene expression. (D) RT-PCR (35 cycles) of selected tissues from an 8-week-old LT/Sv female. Key as per Fig. 1. (E) RT-PCR for PLCZ1 and control ACTB mRNAs in ten independent human ovarian germline tumour (hOGT) biopsy samples as indicated. t, mouse testis control.