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Figure 2


Fig. 2. Detecting DNA methylation with methylation-sensitive restriction enzymes. (A) A methylated (m) region of genomic DNA digested with HpaII (left) or MspI (right). Smaller fragments are discarded (red X), enriching for methylated DNA in the HpaII-treated sample, relative to the MspI-treated. (B) Genomic DNA is treated with McrBC, which cuts methylated (CH3) DNA. Smaller fragments are discarded, enriching for unmethylated DNA. There are many variations on these techniques (see Khulan et al., 2006; Lippman et al., 2004; Schumacher et al., 2006; Tompa et al., 2002).