Fig. 5. Larval body wall muscle phenotypes of Poxm and l(1)sc single and double mutants, and of Poxm mutants rescued by early Poxm. (A-D) The somatic muscle patterns of Poxm^R361 embryos (A), Poxm^R361 embryos rescued by two copies of the um1-2-Poxm transgene (B), of Df(1)sc¹⁹/Df(1)sc¹⁹ or Y (C), and Df(1)sc¹⁹/Df(1)sc¹⁹ or Y; Poxm^R361 (D) embryos and of y w control embryos (not shown) were analyzed at stage 16 under bright-field optics in a Zeiss Axioplan 2 microscope after staining with anti-MHC antiserum, dissection along the ventral midline, and removal of internal tissues. Each muscle plotted on the abscissa was scored for absence (red), abnormality (yellow), duplication (purple), or wild-type appearance (green) in each of 108 (A-C) or 168 (D) hemisegments in abdominal segments A2-A7, and the resulting fractions were plotted on the ordinate. Muscles of the y w control embryos were usually normal in all 108 hemisegments scored, with the occasional absence, duplication or abnormality of a single muscle. (E) The fractions of normal muscles shown in A-D are plotted for Poxm mutants rescued by early Poxm (purple), Poxm (red) and l(1)sc (yellow) single mutants, and for l(1)sc; Poxm (green) double mutants, whereas muscles are ordered along the abscissa with decreasing abnormality of Poxm mutant phenotypes.