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Figure 3


Fig. 3. Transgene expression and worm cross-section. Fluorescent (top) and Nomarski (bottom) images of animals carrying CAM-1::GFP translational fusions. Anterior, left; posterior, right. (A) CAM-1::GFP driven by the cam-1 promoter. Membrane-localized expression is seen here in the ventral cord neurons (arrowheads) and VPCs (arrows). (B) Pmyo-3::CAM-1::GFP is expressed in body wall muscle (arrow). (C) Pdpy-8::CAM-1::GFP is expressed in the hypodermis. Arrows point to hypodermal seam cell nuclei. (D) Pfos-1a::CAM-1::GFP is expressed in the AC (arrow). (E) Psnb-1::CAM-1::GFP is expressed in nervous tissue. Expression shown here is in VCNs (arrowheads). (F) Psur-2::CAM-1::GFP is expressed in the VPCs (arrows) and in a few VCNs. (G) Plst-1::CAM-1::GFP is expressed in the VPCs (arrows). (H) Schematic cross-section of C. elegans hermaphrodite at the vulva. Major tissues are labeled, hatched areas represent sites of cwn-1 and cwn-2 expression.