Fig. 6. Expression analyses of VIGS lines. Petals (pe), sepaloid petals (sp), stamens (st), carpeloid stamens (cs) and pooled stamens and carpels (s/c) were dissected out and used for RT-PCR analyses. Expression of PapsACT1, PapsAP3-1, PapsAP3-2, PapsPI-1 and PapsPI-2 in each of the six VIGS backgrounds and in wild-type flowers is shown. RT-PCR was also used to demonstrate the presence of the TRV2 vector insert in each transformation. PapsACT1 served as an amplification control.