Fig. 3. Inhibition of heparanase function by Ab733 is specifically rescued by FGF10. SMGs were cultured for 48 hours with 1 µl of function-blocking Ab733 (IC₅₀ number of end buds) and increasing doses (see Materials and methods for concentrations) of exogenous FGF1 (10 ng/ml), FGF2 (10 ng/ml), FGF7 (100 ng/ml), FGF10 (100 ng/ml) and HB-EGF (20 ng/ml) were added. The number of buds was expressed as a ratio of the number of buds at 48 hours/number of buds at 1 hour (T48/T1). At least five SMGs per condition were used and the experiment was repeated at least three times. ANOVA compared with the Ab733 alone, ^**P<0.01.