Fig. 5. Expression of HyBra1 and HyBra2 during regeneration in the presence or absence of cycloheximide. Animals were pretreated for 15 minutes in 5 µg/ml cycloheximide, decapitated and allowed to regenerate for the times indicated. The first two hours of regeneration were carried out in the presence of the cycloheximide. (A-C) HyBra1 expression under control conditions. (D-F) The onset of HyBra1 is not affected by cycloheximide. HyBra2 expression in cycloheximide-treated animals (J-L) starts later than in control animals (G-I).