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Figure 2


Fig. 2. Fluorescence images of transverse sections from gonads of chick embryos treated with lindane. (A) E8.5 (HH35) embryo with complete situs inversus. The gonads display inversion of the L-R differences within the epithelium as shown by fibronectin (Fn) and DMRT1; DAPI staining of DNA (blue). (B) E7 (HH31) ZZ embryo with gut heterotaxia double stained for DMRT1 and fibronectin, or for N-cadherin (N-Cad). (C) E7 (HH31) ZW embryo with gut heterotaxia double stained for ER{alpha} and LHX9. In B and C, both left and right gonads display a left pattern consistent with development as left isomers. L, left; R, right. Scale bar: 50 µm.