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Figure 4


Fig. 4. Sections from three samples infected with RCAS-Pitx2a virus at HH8-10, screened at E7 (HH31) for expression of Pitx2 in the right gonad and analysed with the battery of asymmetry markers. (A,B) ZW and ZZ chick embryos with bilateral expression of Pitx2. The gonads are left isomers as shown by staining with DMRT1, fibronectin (Fn), N-cadherin (N-Cad) (white line demarks the epithelium-medulla border), and ER{alpha} plus LHX9. (C) ZZ embryo with very patchy expression of Pitx2 in the right gonad: its phenotype is ambiguous, with some epithelial DMRT1 and localised deposits of Fn, but some areas of the epithelium have flattened (arrows). (D) E7.5 (HH31-32) embryo with gonadal left isomerism following RCAS-Pitx2a infection. Pitx2 expression is detected using either a probe for all Pitx2 isoforms or one specific for Pitx2c. L, left gonad; R, right gonad. Scale bars: white, 50 µm; black, 100 µm.