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Figure 6


Fig. 6. Expression of L-R identity markers in gonads from chick embryos electroporated with RCAS-Pitx2c and GFP DNAs at HH15-16 and screened at E7-8 (HH31-34) for GFP expression. (A-C) Sections of the gonads from three infected embryos (A,B,C are ZZ, ZW and ZW, respectively) showing the epithelial expression of DMRT1 (DM) and accumulation of fibronectin (Fn) found along the A-P axis (Ai,Bi,Ci). These samples also express epithelial ER{alpha} (ER) to different degrees (Aii,Bii,Cii). The induction of epithelial ER{alpha} in the right gonad is independent of the sex as it is also present in the male embryo (Aii). HA-tag antibody staining shows a partial colocalisation between PITX2 and ER{alpha} (Bii). Double-staining for ER{alpha} and LHX9 (LH) shows colocalisation within the cortical layer of the infected right gonad (Cii). (D) High levels of ectopic PITX2 induce the formation of `humps' at the surface epithelium (white arrows) and lack of a proper border between gonad and mesentery (i). The epithelial cells within the humps still express ER{alpha} plus LHX9 (ii), DMRT1 (i,iii) and accumulate high levels of fibronectin (iii). Scale bars: 50 µm.