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Figure 7


Fig. 7. Analysis of cortical differentiation in the right gonad of chick embryos electroporated with RCAS-Pitx2c at HH15-16 and screened at E12 (HH38). (Top row) Transverse sections along the A-P axis of one right gonad containing germ cells clusters (DMRT1-positive) surrounded by fibronectin (Fn). (Bottom row) Left-like cortex areas are positive for ER{alpha} plus LHX9 and contain germ cells positive for DMRT1 plus {gamma}H2AX. The HA antibody staining (right) shows widespread expression of ectopic PITX2 found in extensively infected right gonads. The line marks left-like cortex areas. Scale bars: 50 µm.