Fig. 5. Cdx2 and Nanog variability are not correlated. (A) Cdx2 and Nanog at defined stages of development. For every embryo the counterstained DNA is shown in the row below. White and green arrowheads indicate nuclei of interest of inside and outside cells, respectively. Actin (red) marks the cell membranes. Asterisks indicate blastomeres with only minimal contact to the outside. All images are single optical sections. Scale bar: 20 µm. (B) Scatter plots showing the normalized mean fluorescence intensity of Cdx2 relative to Nanog in individual blastomeres. Each dot represents one blastomere; different colors represent different embryos. Red lines mark the background fluorescence level.