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Figure 2


Fig. 2. Local redistribution of Dorsal as nuclei proceed through mitosis in Drosophila. (A) A lateral view of the transition between relative nuclear inclusion and exclusion (for orientation, see arrow in fourth panel of Fig. 1A). On the dorsolateral side, Dorsal transiently enters nuclei that previously excluded it at the start of mitosis (early prophase) and shortly afterward redistributes in a diffuse relatively uniform pattern (telophase, bottom panel). (B) A side view of the ventral surface showing a transient particulate distribution at the plasma membrane surface at the end of mitosis (white arrow). (C) A surface view (image inverted), showing the distribution of Dorsal-GFP in the particulate compartment on the ventral side (VENTRAL). Dorsal-GFP is never observed in a particulate compartment on dorsal plasma membranes, where nuclei do not take up Dorsal (DORSAL). (D) Dorsal is present within late ventral nuclei diffusely in the nucleoplasm and enriched in the chromosomal compartment.