Fig. 6. Sens and Otd regulate R8 opsin genes in vitro. (A) Table of potential Sens binding sites in Rh5 (-239 to +50) and Rh6 (-555 to +121) promoters. Underlined sequences represent overlapping Otd-binding K50 sites (Tahayato et al., 2003). Promoter diagrams represent binding sites for Otd (blue), Pros (pink) (Cook et al., 2003), or Pax6 (gray) (Papatsenko et al., 2001). (B) EMSA with Rh5 (-239 to +50) and Rh6 (-555 to +121) promoters and 0, 50 and 500 ng His-SensZF. (C-E) Relative luciferase activity of pGL3 or pGL3 with Rh3, Rh4, Rh5 or Rh6 promoters in S2 cells transfected with pAc, pAc-Otd and/or pAc-Sens. (C) ^*, P<0.01 and ^**, P<0.001, compared with pAc alone. (D) ^*, P<0.01 and ^**, P<0.001, compared with Otd alone. (E) ^*, P<0.005 and ^**, P<0.001, compared with Otd alone.