Development -- Hassler et al. 134 (23): 4255 Figure IG5

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Fig. 5. Krm binds to LRP6 specifically and directly. (A,A') Co-IP assays of HEK293T cell lysates transfected with krm1-V5 (A) or krm2-V5 (A') and the indicated constructs. Co-IPs were performed with anti-flag antibody and analyzed by SDS-PAGE and western blotting. (B) In vitro binding assay with secreted recombinant proteins as indicated. IPs were performed with anti-V5 antibody and analyzed by SDS-PAGE and western blotting. Upper panel, protein expression. Middle and lower panels, IPs.