Fig. 7. LRP6 protein is reduced in neural crest of Krm2 morphants. (A) Confocal immunofluorescence analysis of Xenopus NC and animal cap (AC) explants. For NC explants, two-cell-stage embryos were injected with 2 ng LRP6 MO, 3 ng Krm2 MO-1 or 7.5 ng Krm2 MO-2 or CoMO per blastomere. Explants were dissected at stage 14-15, immunostained with anti-ß1-Integrin and anti-LRP6 antibodies, and analyzed by confocal laser scanning microscopy. For AC explants, eight-cell-stage embryos were injected animally with 0.025 ng LRP6 MO, 4 ng Krm2 MO-1 or 15 ng Krm2 MO-2 or CoMO. Explants were dissected at stage 9-10 and analyzed like NC explants. (B) Quantification of relative LRP6 protein levels. The graph shows the ratio of ß1-Integrin (R; red in A) to LRP6 (G; green in A) signal intensities. Numbers in bars indicate the number of analyzed explants. Hoechst-stained nuclei are blue. Scale bar: 50 µm.