Fig. 3. AGO1 is intrinsically required for the establishment and maintenance of
GSCs in Drosophila. Ovaries dissected from flies {hs-flp;
frtG13,/frtG13, ubi-gfp} without heat shock (A), {hs-flp;
frtG13,/frtG13, ubi-gfp} at day 2 AHST (B), {hs-flp;
frtG13,/frtG13, ubi-gfp} at day 10 AHST (C), {hs-flp; frtG13,
Ago1k08121/frtG13, ubi-gfp} at day 2 AHST (D),
and {hs-flp; frtG13, Ago1k08121/frtG13, ubi-gfp}
at day 10 AHST (E,F) were stained with anti-GFP (green) and
anti-Hts (red) antibodies. GFP-negatively marked GSCs and cysts were indicated
by arrows and arrowheads, respectively. Gonads were dissected from
{hs-flp; frtG13,/frtG13, ubi-gfp} (G,G') and
{hs-flp; frtG13, Ago114/frtG13,
ubi-gfp} (H,H') with constitutive heat-shock
treatment at hour 3 after pupa formation were stained with anti-GFP (green)
and anti-Hts (red) antibodies. G' and H' were the amplified images
from G and H, as indicated, respectively. A putative GSC with negatively
marked GFP (an anterior germ cell in a germarium) is indicated by an arrow. A
posterior germ cell with negatively marked GFP is indicated by an arrowhead.
Scale bar: 10 µm.