Fig. 4. Two components of the miRNA pathway, AGO1 and Loqs, are not required for bam silencing in Drosophila. Ovaries dissected from flies {hs-flp; frtG13, Ago1^k08121/frtG13, ubi-gfp} with heat-shock treatment were stained with anti-BamC (red). Two wild-type GSCs that are GFP-positive are negative for BamC (indicated by an arrow) (A), and a negatively marked GFP GSC, indicated by an arrow, was also BamC-negative (B). Ovaries dissected from P{bamP-GFP} (C), loqs; P{bamP-GFP} (D), bam, P{bamP-GFP} (E), loqs; bam, P{bamP-GFP} (F) were stained with anti-GFP (green) and anti-Hts (red) antibodies. GSCs with negative GFP expression are indicated by arrows. Scale bar: 10 µm.