Fig. 7. Phenotypic analyses of Nog mutant mice. (A,B) Lateral view of the tailbud in wild-type and Nog mutant mice at E10.0. Scale bar: 800 µm. (C,D) Hematoxylin and Eosin staining of tailbud sections of wild-type and Nog mutant mice. Scale bar: 100 µm. (E,F) High magnification of the boxed region in C,D. (G,H) Immunostaining of pSmad (red) and E-cadherin (green). Note that pSmad signals were detected in the prospective VER region of Nog mutant mice, and that E-cadherin expression was negative in the same region (arrowheads). Scale bar: 50 µm. (G',H') High-magnification view of boxed area in G and H. Note that the pSmad signal is localized in the nuclei. (I,J) Immunostaining of laminin. (K,L) Double staining of mSnail (blue) and E-cadherin (brown). Note that mSnail was detected in the region without E-cadherin expression in Nog mutant mice (arrowheads). Scale bar: 50 µm. (M,N) Distribution of DiI-labeled cells after 24 hours in culture. (M',N') DiI-labeling at the ventral surface ectoderm of the tailbud. Scale bar: 100 µm.