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Figure 1


Fig. 1. fra and Netrin mutants have defects in a subset of commissural neurons. (A-C) Stage 16 embryos stained with mAb BP102 to display all axons and anti-GFP to visualize a subset of commissural neurons, the Egl neurons. Indicated genotypes also contain UAS-TauMycGFP and eagleGal4. Anterior is up. (A) In wild-type embryos, EW and EG neurons cross the midline in every segment (white arrows). (B) EW axons fail to cross the midline in many segments of Netrin mutants (starred arrow). Note the thinning of CNS commissures (arrowhead) when stained with BP102 (magenta). (C) Similar to Netrin mutants, the EW axons frequently fail to cross the midline in fra mutant embryos (starred arrow). Note the thinning of commissures (arrowhead) when stained with BP102 (magenta). (D-F) Cartoons depicting the trajectories of the EW and EG neurons in each of the indicated genotypes.