Fig. 2. Schematic of the Fra cytoplasmic domain indicating conserved motifs. (A) Sequence of the Fra cytoplasmic domain. Red lines over the sequence indicate the conserved P1, P2 and P3 motifs as defined by Kolodziej et al. (Kolodziej et al., 1996). These sequences were deleted in the constructs shown in B. Orange lines under the sequence indicate the four PXXP motifs found in the Fra cytoplasmic domain. The blue line delimits the region deleted in the construct P3 1/2; this construct leaves the critical Fak-binding residues, defined in vertebrate DCC, intact. (B) Schematic diagram of some of the constructs used in this study. Each construct carries a six-Myc epitope at its carboxyl terminus for comparison of expression levels and localization. Ig, immunoglobulin; FN3, fibronectin type III.