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Figure 4


Fig. 4. The P3 motif is not required for Frazzled multimerization. (A,B) Co-immunoprecipitation of Myc and HA-tagged Frazzled constructs expressed in Drosophila S2R+ cells. Lysates were immunoprecipitated with either anti-Myc antibody (A, and upper left in B), or anti-HA antibody (upper right B). (A) Myr-Fra-Myc co-immunoprecipitates with HA-tagged Fra constructs, including one lacking the P3 motif (lane 3). Fra{Delta}C-HA serves as a control (lane 5). (B) A P3 motif is not required in either construct for interaction, as Myr-Fra{Delta}P3-Myc binds to {Delta}P3 constructs as efficiently as to those containing wild-type cytoplasmic domains (compare lanes 2, 5, 7 and 10 to lanes 1, 4, 6 and 9).