Fig. 5. Nau and Col separately and synergistically activate ectopic col transcription in specific subsets of muscles. (A) P2.6cl expression in the DA3 muscle in stage 15 wt embryos, visualised by β-gal antibody staining. (B) rp298Gal4-driven Col expression of in all FCs activates P2.6cl in a subset of somatic muscles cells, activation being most robust in the VL1 muscle. Nau expression (C) is unable to activate ectopic P2.6cl expression, except for, sporadically, the DA2 muscle. (D) Together, Col and Nau activate P2.6cl expression in a large number of somatic muscles in addition to VL1. A schematic representation of the abdominal muscle pattern is shown of the right side of each panel to indicate the P2.6cl expressing muscles. The DA3, DA2 and VL1 muscles are designated by an arrowhead, a dot and an arrow, respectively.