Fig. 2. Roles of fusion proteins in actin remodeling. Lateral views of stage 14 rp298-lacZ embryos stained with phalloidin to label F-actin (red) and antibody against β-galactosidase to label FCs/myotubes (blue), except E, which is stained with an antibody against Slouch to label a subset of FCs/myotubes (blue). Actin foci are indicated by arrowheads. Scale bar: 5 µm. One optical slice is shown for each mutant. For details of focus size determination, see Materials and methods and Fig. S2 in the supplementary material. (A) Wild type. (B,C) Class 1: no, or few foci. (B) sns^XB3 embryo. (C) rols^T627 embryo. A focus of wild-type size is occasionally seen (arrowhead). (D) Class 2: wild-type actin foci: loner^T1032 embryo. (E-H) Class 3: enlarged actin foci. (E) Rac1^J11, Rac2, mtl embryo. (F) kette^J4-48 embryo. (G) blow¹ embryo. (H) mbc^C1 embryo.