Fig. 3. Localization of fusion machinery with actin focus. Lateral views of stage 14 rp298-lacZ embryos stained with phalloidin to label F-actin (red) and antibodies against β-galactosidase to label FCs/myotubes (blue), and Sns (green, A-A''), Rols (green, B-B''), Loner (green, C-C''), Rac1 (green, D-D''), Kette (green, E-E''), Blow (green, F-F'') or Mbc (green, G-G''). Channels are shown separately and then merged. Sns, Rols, Kette, Blow and Mbc protein colocalize with F-actin foci (arrowheads, A,B,E,F,G), whereas Loner does not (C). Rac partially overlaps with the F-actin foci (D). Scale bar: 5 µm.