Fig. 6. Increased BDNF expression in the cortical neuroepithelium promotes precursor proliferation and enhances neurogenesis in mouse. Cortices were electroporated with EGFP and BDNF or the empty vector, and analyzed at 3 days post-electroporation. (A-C) Quantification of the percentage of (A) EGFP, Ki67-positive cells in the VZ/SVZ, (B) EGFP-positive cells within the cortical mantle and (C) EGFP, HuD-positive cells. n=eight each, five to six sections/embryo. (D) Confocal micrographs of coronal sections immunostained for HuD (red) and EGFP (green). The left panels of each pair are HuD staining, and the right panels are merges of HuD and EGFP staining. Arrows indicate a cluster of EGFP-negative, HuD-positive cells. Error bars indicate s.e.m. Scale bar: 100 µm. ^*P<0.05; ^***P<0.001. V, ventricle.